Intra-cytoplasmic Sperm Injection (ICSI) ICSI is a laboratory procedure developed to help infertile couples undergoing in vitro fertilization (IVF) due to male factor infertility. ICSI, a form of micromanipulation, involves the injection of a single sperm directly into the cytoplasm of a mature egg (oocyte) using a glass needle (pipette). ICSI also works for cases of unexplained non-fertilization with conventional IVF. Image courtesy https://www.invitra.com/en/ivf-or-icsi/